Identification of a microRNA signature associated with survivability in cervical squamous cell carcinoma
نویسندگان
چکیده
BACKGROUND The aim of this study is to find the potential miRNA expression signature capable of predicting survival time for cervical squamous cell carcinoma (CSCC) patients. METHODS The expression of 332 miRNAs was measured in 131 (Training cohort) and 130 (Validation cohort) patients with CSCC in the Cancer Genome Atlas (TCGA) data portal. The miRNA expression signature was identified by Cox Proportion Hazard regression model to the Training data set, and subsequently validated in an independent Validation set. Kaplan-Meier curves and the receiver operating characteristic analyses of 5 years were used to access the overall survival of miRNA signature. MiRNA signature-gene target analysis was performed, followed by the construction of the regulatory network. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis were used to explore the function of target genes of miRNA signature. RESULTS A 2-miRNA expression signature of hsa-mir-642a and hsa-mir-378c associated with survivability was identified in CSCC. Both of them had a significant diagnostic and prognostic value of patients with CSCC. A total of 345 miRNA signature-target pairs were obtained in the miRNA signature-gene target regulatory network, in which 316 genes were targets of has-mir-378c and has-mir-642a. Functional analysis of target genes showed that MAPK signaling pathway, VEGF signaling pathway and endocytosis were the significantly enriched signal pathways that covered most genes. CONCLUSIONS The 2-miRNA signature adds to the prognostic value of CSCC. In-depth interrogation of the 2-miRNAs will provide important biological insights that finding and developing novel molecularly prediction to improve prognosis for CSCC patients.
منابع مشابه
Rate of Occult Cervical Lymph Node Involvement in Supraglottic Squamous Cell Carcinoma
Introduction: To assess the rate of cervical lymph node involvement in patients with supraglottic squamous cell carcinoma (SCC) with no lymph node in clinical assessments and radiological studies. Materials and Methods: Fifty-six patients who underwent elective dissection of the cervical lymph node of the second through fourth level were enrolled, and pathologic evaluation of the dissected lymp...
متن کاملDiagnostic Accuracy of CT Scan for Detection of Cervical Lymph Node Metastasis in Oral Squamous Cell Carcinoma in Comparison with Histopathological Analysis After Neck Dissection
Objectives: Presence/absence of cervical lymph node metastasis plays a critical role in prognosis and survival of patients with oral squamous cell carcinoma (SCC). This study was designed to assess the diagnostic accuracy of computed tomography (CT) scan for detection of cervical lymph node metastasis in oral SCC in comparison with histopathological analysis after neck dissection Methods: In...
متن کاملA case of squamous cell carcinoma of the skin subsequent to a subcutaneous foreign body
Squamous cell carcinoma (SCC) of the skin is one of the mostcommon non melanoma skin cancers (NMSC), along withbasal cell carcinoma (BCC). Besides ultraviolet radiation,exposure to industrial agents, ionizing radiation, and areas ofchronic inflammation are associated with the development ofSCC. Squamous cell carcinoma may also be associated withforeign bodies. We report a rare case of cutaneous...
متن کاملIdentification of p53 Gene Mutations among Iranian Patients Involved with Esophageal Squamous Cell Carcinoma: the Efficiency of the Two Different Methods
متن کامل
A Comparative Study of CT Scan and MRI in The Assessment of Squamous Cell Carcinoma of The Oral Cavity and Its Cervical Lymph Node Metastasis
Introduction: Squamous Cell Carcinoma (SCC) is the most common head and neck malignancy. To decrease the side effects of treatment and mortality from disease relapse, careful staging for the proper treatment plan is necessary. The main purpose of this prospective study is to compare the diagnostic value of Computed Tomography (CT) and Magnetic Resonance Im-aging (MRI) in oral SCC and its lymph ...
متن کامل